complete antigen

Preparation and analysis of complete antigen for okadaic acid

海洋毒素大田软海绵酸完全抗原的制备与分析

Synthesis of Complete Antigen of Microcystin-LR and Its Antibody Production

微囊藻毒素-LR完全抗原的设计及制备

Synthesis of Clenbuterol Hydrochloride Complete Antigen and Optimization of its Conjugation Conditions

盐酸克伦特罗完全抗原的合成及其偶联工艺的优化

Study on Preparation of Complete Antigen of Sulfamonomethoxine

磺胺间甲氧嘧啶全抗原的制备研究

Synthesis of a complete antigen of thyroxine and preparation of its antiserum

甲状腺素全抗原的合成及其抗血清的制备

Objective Characterizate the complete antigen of the chlorogenic acid .

目的制备绿原酸完全抗原，对完全抗原进行鉴定。

Synthesis of a Complete Antigen of Chloramphenicoles and Preparation of Its Monoclonal Antibody

氯霉素类抗生素全抗原合成及单克隆抗体制备的初步研究

CDV is sensitive to the physical and chemical factors , and it is difficult to obtain the complete antigen .

由于犬瘟热病毒对理化因素敏感，难以通过常规病毒纯化的方法获得包被抗原。

The UV scanning result showed that OVA and conjugate had different wave type . So it indicated the existence of complete antigen .

紫外扫描显示，偶联物与OVA的波型不同，可初步判断完全抗原的存在。

Protein microarray analysis also showed that specific reaction occurred between OTA McAb and conjugate , suggesting the existence of complete antigen .

同时蛋白质芯片检测结果显示，偶联物与OTAMcAb之间发生了特异性反应，提示完全抗原的存在。

In a word , the whole heat-killed staphylococcus aureus or carbohydrate-binding protein can be complete antigen .

从而了解到完全抗原只能用灭活金黄色葡萄球菌或者纯化的荚膜多糖结合蛋白质来实现。

Methods : The synthesized BNP was conjugated to bovine serum albumin ( BSA ) by EDC coupling procedure to form complete antigen ( BNP-BSA ) .

方法：合成的BNP与牛血清白蛋白（Bovineserumalbumin，BSA）以碳化二乙胺（EDC）法偶联，制备BNP-BSA完全抗原。

In this study penicillin hapten was respectively coupled with BSA and OVA to synthesize PEN-EDC-BSA and PEN-EDC-OVA complete antigen applying CDI .

本研究分别以牛血清白蛋白（BSA）、卵清蛋白（OVA）为蛋白质载体，采用碳二亚胺法（CDI）将半抗原青霉素钠（PEN）分别与BSA、OVA偶联，合成了完全抗原PEN-EDC-BSA和PEN-EDC-OVA。

Added amount of EDC controlled in 6 ~ 9mg . The New Zealand rabbits were immunized with complete antigen of combined ratio of 16:1 , the antiserum 6.55 × 106 was obtained .

以结合比为16：1的完全抗原免疫新西兰白兔，获得了效价达到6.55×106兔抗血清。

Conjugating Sal to bovine serum albumin ( BSA ) and ovi albumin ( OVA ) by succinic anhydride and carbodiimide transformed Sal , an half antigen , into complete antigen .

本实验采用琥珀酸酐和碳二亚胺法将沙丁胺醇偶联到牛血清白蛋白和卵清白蛋白上，成为完全抗原Sal-HS-BSA和Sal-HS-OVA。纯化后进行检测，确认沙丁胺醇已经连接到蛋白质上。

Good quality polyclonal antibody against Microcystin-LR ( MC-LR ) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR . Indirect ELISA shows the titer of this antibody is more than 1.5 × 10 ~ 5 ;

通过对新西兰大白兔免疫自制的微囊藻毒素-LR（Microcystin-LR，MC-LR）完全抗原BSA-MC-LR，获得了质量较好的抗MC-LR的多克隆抗体，间接ELISA表明抗体的效价能达到1.5×105；

The completed antigen was dialyzed and identified by SDS-PAGE , UV-scanning and bio-mass spectrometry . The result show that the coupling ratio between MC-LR and BSA of complete antigen in average was over 5 , and it is sufficient to immunity .

中间产物和偶联产物分别经固相萃取和透析纯化后，经SDS凝胶电泳、紫外扫描及生物质谱技术鉴定，结果表明MC-LR与BSA的平均偶联比能达到5以上，满足了进一步免疫的要求。

The affinity constants for complete antigen and Fe porphyrin were 3.65 × 10 - 9 and 8.20 × 10 - 8 respectively . The activity of antibody Fe porphyrin complex with catalyzed reaction was 46.875 ? U / mg , which compared with natural HRP was 18.99 % .

该抗体对抗原和铁卟啉的亲和常数为3.65×10-9和8.20×10-8.抗体铁卟啉复合物催化反应时，得到比活力为46.875U/mg，为天然HRP的18.99%。

Conclusions The red blood cells may have grossly complete D antigen expression in the DEL individuals carrying RHD1227A alleles .

结论携带RHD1227A等位基因的中国汉族D放散型个体红细胞膜可能表达基本完整D抗原。